연구분야

I am interested in studying predation of bacterial strains by Bdellovibrio-and-like-organisms (BALOs). These incredible little bacterial strains actually attack and eat other bacteria and can be found in many environments, including the soil and ocean.

Reseach in my lab will include the characterization of these bacterial strains and their predation with a focus on:

– The responses of the bacterial prey to predation, including resistance

– Genetic, transcriptional and proteomic analyses of BALOs

– Microarray and RT-PCR analyses and biosensor development

– Various applications of BALOs in industrial/medical facilities

– Environmental applications and clean-up

A second area of research will involve improving the generation of biofuels, including hydrogen and butyric acid, with fermentative bacterial strains. Some of the topics being addressed include:

– Characterization of different bacterial strains and their ability to produce biofuels

– Genetic modification of these strains to improve production

– Mixed culture/consortia studies and characterization

– Toxicogenomic analyses using microarrays and RT-PCR

대표 연구실적

Mitchell RJ, Kim T, Lee SK, Ghim C-M. 2011. Microbial Linguistics: Perspectives and Applications of Microbial Cell-to-Cell Communication. BMB Reports 44(1):1-10

Yaguchi T, Lee S, Choi WS, Kim D, Kim T, Mitchell RJ, Takayama S. 2010. Micropatterning Bacterial Suspensions Using Aqueous Two Phase Systems. Analyst 135:2848-2852

Ghim C-M, Lee SK, Takayama S, Mitchell RJ. 2010. The art of reporter proteins in science: past, present and future applications. BMB Reports 43(7):451-460

Ghim C-M, Kim T, Mitchell RJ, Lee SK. 2010. Synthetic biology for biofuels: Building designer microbes from the scratch. (Review Article) Biotechnology and Bioprocess Engineering, 15(1):11-21

Mitchell RJ, Kim JS, Jeon BS, Sang BI. 2009. Continuous hydrogen and butyric acid fermentation by immobilizedClostridium tyrobutyricum ATCC 25755: Effects of the Glucose Concentration and  Hydraulic  Retention Time.Bioresource Technology 100:5352-5355

Mitchell RJ, Gu MB. 2006 Characterization and optimization of two methods in the immobilization of  12 bioluminescent strains. Biosens Bioelectron. 22(2):192-199.

Lee JH, Mitchell RJ, Gu MB. 2007 Chemical-specific continuous biomonitoring using a recombinant bioluminescent bacterium DNT5 (nagR-nagAa::luxCDABE). J. Biotechnol. 131:300-304

Mitchell RJ, Hong HN, Gu MB. 2006 Induction of kanamycin resistance gene of plasmid pUCD615 by benzoic acid and phenols. J. Microbiol. Biotechnol. 16 (7):1125-1131

Lee JH, Mitchell RJ, Kim BC, Cullen DC, Gu MB. 2005 A Cell Array Biosensor for Environmental Toxicity Analysis. Biosensors and Bioelectronics 21(3):500-507

Mitchell RJ, Ahn JM, Gu MB. 2005 Comparison of Photorhabdus luminescens and Vibrio fischeri lux fusions to study gene expression patterns. J. Microbiol. Biotechnol. 15(1): 48-54

Mitchell RJ, Gu MB. 2005. Construction and evaluation of nagR-nagAa::lux fusion strains in the biosensing for salicylic acid derivatives.  Appl. Biochem. Biotechnol.  120(3):183-98

Ahn JM, Mitchell RJ, Gu MB. 2004. Detection and classification of oxidative damaging stresses using recombinant bioluminescent bacteria harboring sodA ::, pqi ::, and katG ::luxCDABE fusions.  Enzyme Microbial Technol. 35(6-7):540-544

Lee JH, Mitchell RJ, Gu MB 2004. Enhancement of the multi-channel continuous monitoring system through the use ofXenorhabdus luminescence lux fusions. Biosens. Bioelectronics. 20(3):475-481

Mitchell RJ, Gu MB. 2004. Construction and characterization of novel dual-stress-responsive bacterial biosensors.  Biosens. Bioelectron. 19(9):977-985

Mitchell RJ, Gu MB. 2004. An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage.  Appl. Microbiol. Biotechnol. 64(1):46-52

Gu MB, Mitchell RJ, Kim BC. 2004. Whole cell-based biosensors for environmental monitoring and application. Adv. Biochem. Eng. Biotechnol. 87:269-305

Gil GC, Mitchell RJ, Chang ST, Gu MB. 2000. A biosensor for the detection of gas toxicity using a recombinant bioluminescent bacterium.  Biosens. Bioelectron. 15(1- 2):23-30

Gu MB, Mitchell  RJ, Kim JH. 2000. Continuous monitoring of protein damaging toxicity using a recombinant bio-luminescent Escherichia coli.  ACS Symposium Series: Recent Advances in Chemical Sensors and Biosensors for Environmental Monitoring. 762:185-196